Comparative Studies on Synthetic and Agricultural Product on Lysine Production by Alcaligenes aquatilis
C. B. Nwokolo *
Department of Applied Microbiology and Brewing, Nnamdi Azikiwe University Awka, Anambra State, Nigeria.
N. N. Uchefuna
Department of Applied Microbiology and Brewing, Nnamdi Azikiwe University Awka, Anambra State, Nigeria.
I. A. Ekwealor
Department of Applied Microbiology and Brewing, Nnamdi Azikiwe University Awka, Anambra State, Nigeria.
C. T. Ezeh
Department of Applied Microbiology and Brewing, Nnamdi Azikiwe University Awka, Anambra State, Nigeria.
C. C. Ezemba
Department of Microbiology, Renaissance University, Ugbawka, Enugu State, Nigeria.
*Author to whom correspondence should be addressed.
Abstract
Production of lysine by Alcaligenes aquatilis from agricultural sub-products (banana and soybean) was compared to glucose and ammonium sulphate as a carbon and nitrogen source. Ammonium sulphate was constant as a nitrogen source when the two carbon sources were investigated and glucose constant as a carbon source when the nitrogen sources were investigated. The production of lysine was examined quantitatively by acidic ninhydrin method. The results showed that banana and soybean improved the maximum lysine yield (1.158 mg/ml and 1.279 mg/ml) for the fermentation period of 96 hrs.
Keywords: Lysine, ninhydrin, glucose, banana, soybean, ammonium sulphate, Alcaligenes aquatilis