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Sweet potato (SP) popularly known as Ipomoea batatas, has played an important role as an energy and a phytochemical source in human nutrition and animal feeding as well as herbal medicine to treat inﬂammatory and infectious oral diseases in Nigeria. This research aims to evaluate the secondary metabolites (phytochemical), antioxidants and antimicrobial activities as well as proximate analysis and to determine the minerals content of sweet potato ethanolic leaf extracts. The screenings were performed for triterpenes, steroids, alkaloids, anthraquinones, coumarins, ﬂavonoids, saponins, tannins and phenolic acids. The color intensity of the precipitate formation was used as analytical responses to these tests. The antioxidant capacity was evaluated and antimicrobial activity was done by agar well diffusion method. The phytochemical screening showed the presence of secondary metabolites. Alkaloids, saponin, cardiac glycosides, tannin, terpenoidand steriod respectively with the following values 13.649%, 2.545 mg/g, 8.271 mg/g, 4.726 mg/g, 6.888 mg/g and 0.276%. Flavonoids were 0.852 while negative results were obtained for phlobatannin and anthraquinone. Proximate analysis of SP leaf, moisture content(MC), crude fat (CF), crude protein (CP),ash content (ASH), cured fat (CFAT) and carbohydrate(CHO) as 32.98%, 0.725%, 14.953%, 5.863%, 0.319% and 45.16%, respectively. Ca, Mg, Fe, Na and K were the mineral contents determined for SP. The zones of inhibition of bacterial growth at different concentrations (100 mg/ml, 50 mg/ml, 25 mg/ml and 12.5 mg/ml) of SP leaf extracts showed the highest antibacterial activity to Salmonella typhi with 25 mm zones of inhibition at 100 mg/ml and lowest activity to Listeria monocytogens, Staphylococcus aureus, Vibrio cholea and Pseudomonas aeruginosa all with 20 mm zones of inhibition and 12.5 mg/ml. However, the leaf extracts had the lowest antimicrobial activity to Enterrococcus faecalis, with 7 mm diameter. SP leaves demonstrated the presence of secondary metabolites with potential biological activities. Therefore it can be concluded that bioactive components and antimicrobial activity of sweet potato leaf ethanol extract against bacteria show its medicinal value and supports the widespread use of the plant as local remedy for a variety of ailment.
Loebenstein G, Thottappilly G. The sweet potato. Springer Verlag. 2009;391–425
Sakamoto S, Bouwkamp JC. Industrial products from sweet potatoes. In: Sweet potato products: A natural resource for thetropics (Bouwkamp JC, ed.), CRC Press, Boca Raton, Florida. 2015;219–234.
Watt BK, Merrill AL. Composition of foods: raw, processed, prepared in: Agriculture Handbook No. 8, U.S. GovernmentPrinting Office, Washington D.C; 1975.
Yen DE. Sweet potato in historical perspective. In: Sweet Potato. Proceedings of the 1st International Symposium, (Villareal RL, Griggs TD, ed.) Asian Vegetable Research and Development Center Taiwan. 1982;17–30.
Huang DJ, Lin CD, Chen HJ, Lin YH. Antioxidant and antiproliferative activities of sweet potato (Ipomoea batatas [L.] Lam ‘‘Tainong 57’’) constituents. Bot Bull Acad Sin. 2004;45:179–186.
Cereda MP, Franco CML, Daiuto ER, Demiate JM, Carvalho L. Propried adesgerais do amido, Fundac¸a˜oCargill, Campinas, Brasil. 2001;21.
Antia BS, Akpan EJ, Okon PA, Umoren IU. Nutritive and antinutritive evaluation of sweet potatoes (Ipomoea batatas) Leaves.Pak J Nutr. 2006;5:166–168.
Woolfe JA. Sweet potato–past and present. In: Sweet potato: An untapped food resource, Cambridge University Press, Cambridge. 2012;15–40.
Trumbo RP, Ellwood KC. Lutein and zeaxanthin intakes andrisk of age-related macular degeneration and cataracts: an evaluation using the Food and Drug Administration’s evidence-basedreview system for health claims. America Journal of Clinical Nutrition. 2006;84:971–974.
Islam S. Antimicrobial activities of Ipomoea batatas (L.) leaf. J Food Agr Environ. 2008; 6:14-7.
Pochapski MT, Fosquiera EC, Esmerino LA, Santos EB, Farago PV, Santos FA. Phytochemical screening, antioxidant, and antimicrobial activities of the crude leaves’ extract from Ipomoea batatas (L.) Lam. Pharmacogn Magazine. 2011;7:165–170.
Osuntokun Oludare Temitope. Bioactivity and phytochemical screening of Nigerian Medicinal Plants Growing in Ondo and Ekiti State against Bacterial Isolates from Pediatrics Hospital. Journal of Advances in Medical and pharmaceutical Sciences. 2015;4(2):1-14,
Osuntokun Oludare Temitope FA, Olajubu TA, Olorunnipa OA, Thonda. Elemental composition, Eva luation of anti-nutrients, and antioxidant potentials of Morinda lucida, European Journal of Medicinal Plants. 2016;12(4):1-10.
Osuntokun OT. Evaluation of Inhibitory Zone Diameter (IZD), Phyto chemical screening, elemental comp osition and proximate analysis of crude Cleistopholis patens (Benth) on infectious clinical isolates. J Mol Biomark Diagn. 2018;9: 385.
DOI: 10.4172/2155-9929. 1000385,
Oludare Temitope Osuntokun AM, Yusuf-Babatunde OO, Ige, AE Odufuwa. Phytochemical screening and evaluation of antioxidant and proximate properties of Morinda lucida ethanolic extract. Journal of Advances in Medical and Pharmaceutical Sciences. 2016;11(1):1-11.
Singleton VL, Orthofer R, Lamuela- Raventos RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent Methods, Enzymol. 1999;299:152- 178.
Bao JY, Cai M, Sun G, Wang H, Corke. Anthocyanins, flavonoid and free radical scavenging activity of thines baybery (Myrialrubia) extracts and their colour properties and stability. Journal of Agriculture and Food Chemistry. 2005;53: 2327-2332.
Pulido R, Bravo L, Saura-Calixto F. Antioxidant activity of dietary polyphenols as determined by a modified ferric reducing/antioxidant power assay. Journal of Agricultural and Food Chemistry. 2000;48:3396–3402.
Gyamfi MA, Yonamine M, Aaniya Y. Free radical scavenging action of medicinal herbs from Ghana: thonningia sanguine on experimentally induced liver injuries. General Pharm Acology. 1999;32:661– 667.
Jagetia Ganesh Chandra, Rao, Shaival Kamalaksh, Baliga Manjeshwar Shrinath, Babu, Kiran S. The evaluation of nitric oxide scavenging activity of certain herbal formulations in vitro: A preliminary study Phytotherapy Research. 2004;18(7):561-565.
Puntel RL, Nogueira CW, Rocha JBT. Krebs cycle intermediates modulate thiobarbituric acid reactive species (TBARS) production in rat brain in vitro Neurochem. Res. 2005;30:225-235
Re R, Pellegrin N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improve ABTS Radication decolourization assay. Free Rad. Biol. Med. 1999;26:1231-1237.
Halliwell B, Gutte Ridge JM. Formation of a thiobarbituric-acid-reactive substance from deoxyribose in the presence of iron salts: The role of superoxide and hydroxyl radicals FEBS. Lett. 1981;128:347-352.
Oludare Temitope Osuntokun, Bamidele O, Aladejana OM, Ogunlade AO. Evaluation of antimicrobial Activity, synergistic efficacy, qualitative and quantitative phytochemical determination of Alstonia boonei leaf and stem bark on selected clinical isolates. Triple A Research Journal of Multidisciplinary (JMD). 2017;1(1):001-014.
Oludare Temitope Osuntokun, Baraldi Cecilia, Gamberini Maria Cristina. Evaluation of Quantitative Elemental Compositions and antioxidant potentials of Spondias mombin Extracts (Linn): A precursor against infectious diseases. World Journal of Pharmacy and Pharmaceutical Sciences. 2018;3:964-985.
[ISSN: 2278 – 4357]
Makkar HPS, Goodchild AV. Quantification of tannins: A laboratory manual. ICARDA; Aleppo, Syria: 1996.
Brunner D, Oellers N, Szabad J, Biggs WH, Zipursky SL, Hafen E. A gain-of-function mutation in Drosophila MAP kinase activates multiple receptor tyrosine kinase signaling pathways. Cell. 1994;76 (5):875-888.
Harbone J. Phytochemical methods: A guide to modern techniques of plant analysis 3rd Edition. Chapman and Hill, London. 1998;279
Harbone J. Phytochemical methods: A guide to modern techniques of plant analysis. 3rd Edition. Chapman and Hill, London. 1999;279.
Horwitz W. Editor. Official methods of analysis of AOAC Inter national; 17th ed. USA: Association of Official Analytical Communities; 2003.
Hassan MM, Oyewale AO, Amupitan JO, Abdullahi MS, Okon Kwo. B. Preliminary phytochemi cal and antibacterial investigation of crude extracts of the root bark of Detarium microcapum. J. Chem. Soc. Nig. 2004;29:26.
Al-Harrasi A, Al-Rawahi A, Hussain J, Rehman N, Ali L, Hussain H. Proximate analysis of the resins and leaves of Boswellia sacra. Journal of Medicinal Plants Resources. 2012;6(16):3098-3104.
Basnet P, Matsushige K, Hase K, Kadota S, Namba T. Fourdi-O-caffeoylquinic acid derivatives from propolis potent Sweet Potato As Medicinal Food hepatoprotective activity in experimental liver injury models. Biol Pharm Bull. 1996;19:1479–1484.
Afolabi C, Akinmoladun EO, Ibukun EA, Obuotor EM, Farombi EO. Phyto chemical constituent and antioxidant activity of extract from the leaves of O. gratissimum, Scientific Research and Essay. 2007;2(5): 163-166.
Carvalho Basnet P, Matsushige K, Hase K, Kadota S, Namba T. Fourdi-O-caffeoylquinic acid derivatives from propolispotent Sweet Potato As Medicinal Food 7 hepatoprotective activity in experimental liver injury models. Biol Pharm Bull. 1996;19:1479–1484.
Borges EL, Carlos, LM, Silva MAM, Magalhães SM, Gomes F, Pitombeira MH. Efeito da bomba de infusão de soluções sobre o grau de hemólise em concentrado de hemácias Revista Brasi leira de Hematologiae Hemo terapia. 2007;29 (2):149-152.
Dini I, Tenore GC, Dini A. New polyphenol derivative in Ipomoea batatas tubers and its antioxidant activity. J Agric Food Chem. 2006;54:8733–8737.
Kilham C. Tamanu oil: A tropical topical remedy. Herbal Gram. 2004;63:26–31.
Rumbaoa RGO, Cornago DF, Geronimo IM. Phenolic contentand antioxidant capacity of Philippine sweet potato (Ipomoea batatas) varieties. Food Chem. 2009;113:1133–1138.
Runnie I, Salleh MN, Mohamed S, Head RJ, Abeywardena Y. Vasorelaxation induced by common edible tropical plantextracts in isolated rat aorta and mesenteric vascular bed. J. Ethno Pharmacol. 2004;92:311–316.
Ndukwe. Ndukwe, OK, Awo mukwu, D, Ukpabi CF. Compa rative evaluation of phytochemical and mineral constituents of the leaves of some medicinal plants in Abia State Nigeria, International Journal of Academic Research in Progressive Education and Development. 2013;2(3)0
DOI: 10.6007/ IJARPED/v2-i3/148
Available:http://dx.doi.org /10.6007 /IJARPED/v2-i3/148
Brosnan J. Interorgan amino acid transport and its regulation. Journal of Nutrition. 2003;133:2068-2072.
Ekuagbere AO. Chemical, mineral and amino acid composition and functional properties of calabash seed. 2007;24-36.
Olaofe O, Okiribiti BY, Aremu MO. Chemical evaluation of the nutritive value of smooth luffa (Luffa cylindrical) seeds and kernel. Electronic Journal of Environmental, Agricultural and Food Chemistry. 2008;3444-52.
Apata DF, Ologhobo AD. Biochemical evaluation of some Nigerian legume seeds. Food Chemistry. 1994;49:333-338.
Akindahunsi AA, Salawu SO. Phytochemical screening and nutrient-antinutrient composition of selected tropical green leafy vegetables. African Journal of Biotechnology. 2005;4:497-501.
Lintas C. Nutritional aspect of fruits and vegetables consumption options. Mediterraunnes. 1992;19:97-87.
Ekpo MA, Etim PC. Antimicrobial activity of ethanolic and aqueous extracts of Sidaacuta on microorganisms from skin infections. J Med Plant Res. 2009;3(9): 621-624.
Hermes D, Dudek DN, Maria M, Horta LP, Lima EN. In vivo wound healing and antiulcer properties of whitesweet potato (Ipomoea batatas). J Adv Res. 2013;4: 411–415.
Ishida H, Suzuno H, Sugiyama N, Innami S, Tadokoro T, Maekawa A. Nutritive evaluation on chemical components of leaves, stalks and stems of sweet potatoes (Ipomoea batatas Poir). Food Chem. 2000;68:359–367.
Oke JM, Oladosu B, Okunola MC. Sweet potato (Ipomoea batatas) tuber - potential oral antidiabetic agent. Afr J Biomed Res. 1999;2:13–17.
Nwaogu LA, Alisi CS, Ibegbulem CO, Igwe CU. Phytochemical and antimicrobial activity of ethanolic extract of Landolphiao wariensis leaf, African Journal of Biotechnology. 2007;6(7):890-893.
Chang WH, Hu SP, Huang YF, Yeh TS, Liu JF. Effect of purple sweet potato leaves consumption on exercise-induced oxidative stress and IL-6 and HSP72 levels. J Appl Physiol. 2010;109:1710–1715.
Chuku EC, Ugorji JH. Determination of levels of some nutrients and ant nutrients in five selected vegetables in Niger delta. Scienta Africana. 2012;11(1):130-142.
Dini I, Tenore GC, Dini A. Saponins in Ipomoea batatas tubers: Isolation, characterization, quantification and antioxidant properties. Food Chem. 2009; 113411-419.
Ngaski MM. Phytochemical screening and p roxim.ate analysis of Cassia swmea leaves. M.Sc. Dissertation (Unpublished). Submitted to postgraduate school, Usmanu Danfodiyo University, Sokoto; 2006.
Guan Y, Wu T, Lin M, Lin M, Ye J. Determination of pharma cologically active ingredients in sweet potato (Ipomoea batatas) by capillary electrophoresis with electrochemical detection. J. Agric Food Chem. 2004;54:24–28.
Rengarajan S, Rani M, Kumar Esapillai N. Study of ulcer protective effect of Ipomea batatas (L.) dietary tuberous roots(Sweet Potato). Iranian J Pharmacol Therap. 2012;11:36–39.
Olaofe O, Umar YO, Adediran GO. The effect of nematicides on the nutritive value and functional prop.of cowpea seeds. (Vigna unguiculata L. Walp), Food Chemistry. 1994;46:337-341.