Main Article Content
Aims: The purpose of this study was to determine the percentage value of spermatozoa acrosome integrity with the addition of gentamicin and sweet orange essential oil on the tris yolk extender on Simmental bull liquid semen.
Methodology: The material that was used in this research included Simmental bull fresh semen, tris yolk extender, gentamicin, and sweet orange essential oil. Tris yolk extender prepares with Tris (hydroxymethyl) aminomethane (3.32 g), citric acid (1.86 g), fructose (1.37 g), glycerol (6 ml), egg yolk (20 ml), aqua dest (100 ml). The experimental design used in the study was a nonfactorial Complete Randomized Design with five treatments and five replications. The treatments comprised the addition of sweet orange essential oil at 0%, 0.25%, 0.5%, 0.75% and 1% inclusion levels. The parameter observed was the evaluation of the percentage value of acrosome integrity before and after equilibration equilibration.
Results: The results showed that the percentage value of acrosome integrity in spermatozoa linearly increased along with the addition of sweet orange essential oil. The addition of sweet orange essential oil had a very significant effect (P <0.01) on the percentage of spermatozoa acrosome integrity in Simmental bull liquid semen. The percentage of acrosome integrity on the addition of 0% (P0) sweet orange essential oil is 60%, the addition of 0.25% (P1) is 64%,the addition of 0.5% (P2) is 67%, the addition of 0.75% (P3) is 69%, and the addition of 1% (P4) is 73%.
Conclusion: The addition of 1% (P4) sweet orange essential oil can increase the value of acrosome integrity on spermatozoa, which subsequently improve the quality of Simmental bull liquid semen.
Tambing SN, Sutama IK, Sariubang M. Efficacy of concentration of egg yolk in Tris extender with and without seminal plasma on frozen semen quality of Saanen bucks. JurnalIlmu Ternakdan Veteriner. 2009; 13(4):315-322.
Toelihere MR. Inseminasi Buatan Pada Ternak. Angkasa, Bandung; 1993.
El-Sheshtawy RI, Sisy GA, El-Nattat WS. Effects of different concentrations of sucrose or trehalose on the post-thawing quality of cattle bull semen. Asian Pacific Journal of Reproduction. 2015;4(1):26- 31.
Hafez ESE, dan B. Hafez. Reproduction in Farm Animal 7th Ed. Lippincott Williams and Walkins, South Carolina; 2000.
Hernández-Avilés C, Serafini R, Love CC, Teague SR, LaCaze KA, Lawhon SD, Varner DD. The effects of antibiotic type and extender storage method on sperm quality and antibacterial effectiveness in fresh and cooled-stored stallion semen. Theriogenology. 2018;122:23-29.
Zaituni U. Teknologi Inseminasi Buatan dan Transfer EmbrioPadaSapi. Sukabina Press, Padang; 2012.
Nattadiputra S dan S. Munaf. Aminoglikosidadan Beberapa Antibiotika Khusus, Kumpulan Kuliah Farmakologi. 2009;631. Jakarta, EGC.
Fisher K, Phillips CA. Potential antimicrobial uses of essential oils in food: Is Citrus The Answer?.Trends in Food Sci and Technology. 2008;19(3):156-164.
Sitepu SA, Marisa J. Percentage value of membrane integrity and acrosome integrity Spermatozoa in Simmental Liquid Semen with Addition Penicillin and Sweet Orange Essential Oil. In IOP Conference Series: Earth and Environmental Science. IOP Publishing. 2019;3271:012027.
Saacke RG, White JM. Semen quality tests and their relationship to fertility. prooceeding 4th Tech Conf on AI and Reprod NAAB. 1972;22-27.
Xu J, Chaubal SA, Du F. Optimizing IVF with sexed sperm in cattle. Theriogenology. 2009;71(1):39-47.
Wang W, Luo J, Sun S, Xi L, Gao Q, Haile AB, Shi H. The effect of season on spermatozoa motility, plasma membrane and acrosome integrity in fresh and frozen–thawed semen from XinongSaanen bucks. Reproduction in Domestic Animals. 2015;50(1):23-28.
Bilaspuri GS, Bansal AK. Mn2+: A potent antioxidant and stimulator of sperm capacitation and acrosome reaction in crossbred cattle bulls. Archives Animal Breeding. 2008;51(2):149-158.
Srivastava N, Srivastava SK, Ghosh SK, Kumar A, Perumal P, Jerome A. Acrosome membrane integrity and cryocapacitation are related to cholesterol content of bull spermatozoa. Asian Pacific Journal of Reproduction. 2013;2(2):126-131.
Sinha S, Deka BC, Tamulu MK, Borgohain BN. Effect of equilibration period and glicerol level in tris extender of quality of frozen goat semen. Indian Vet. J. 1992;69: 1107-1110.
Dewi ADR. Aktivitas Antioksidan Dan Antibakteri Ekstrak Kulit Jeruk Manis Dan Aplikasinya Sebagai Pengawet Pangan. Jurnal Teknologi & Industri Pangan. 2019;30(1):83-90.
Thuwanut P, Chatdarong K, Techakumphu M, Axner E. The effect of antioxidants on motility, viability, acrosome integrity and DNA integrity of frozen-thawed epididymal cat spermatozoa. Theriogenology. 2008; 70(2):233-240.