Membrane Integrity Test for Spermatozoa in Simmental Bull Liquid Semen Using the Addition of Gentamycin and Sweet Orange Essential Oil in the Extender

Main Article Content

Sukma Aditya Sitepu
Julia Marisa

Abstract

Aims: This study aimed to determine the quality of Simmental Bull liquid semen through laboratory tests, including the percentage value of membrane integrity Spermatozoa with the addition of gentamycin and sweet orange essential oil to tris yolk extender.

Methodology: The material used in this research is Simmental Cattle bull fresh semen, tris yolk extender, gentamicin, and sweet orange essential oil. Tris yolk extender was prepared with Tris (hydroxymethyl aminomethane) (3.32 g), citric acid (1.86 g), fructose (1.37 g), glycerol (6 ml), egg yolk (20 ml), aquades (100 ml). The experimental design used in the study was a nonfactorial Complete Randomized Design with five treatments and five replicates. The treatment given is the addition of sweet orange essential oil 0%, 0.25%, 0.5%, 0.75% and 1%. The parameter observed was the evaluation of membrane integrity before equilibration and after equilibration.

Results: The results showed that the percentage of membrane integrity in Simmental bull liquid semen continues to increase with the addition of sweet orange essential oils. The results showed that the addition of sweet orange essential oil as much as 0% produced a membrane integrity percentage after equilibration of 63%, adding 0.25% resulted in 68%, adding 0.5% yielded 70%, adding 0.75% yielded 75% and adding 1% resulted in 79%.

Conclusion: The best percentage of membrane integrity in Simmental bull liquid semen was the addition of gentamicin and sweet orange essential oil in the tris yolk extender at the level of 1%.

Keywords:
Essential oil, gentamicin, liquid semen, membrane integrity, sweet orange, simmental.

Article Details

How to Cite
Sitepu, S. A., & Marisa, J. (2020). Membrane Integrity Test for Spermatozoa in Simmental Bull Liquid Semen Using the Addition of Gentamycin and Sweet Orange Essential Oil in the Extender. Asian Journal of Advanced Research and Reports, 8(4), 42-46. https://doi.org/10.9734/ajarr/2020/v8i430206
Section
Original Research Article

References

Hernández-Avilés CR, Serafini CC, Love SR, Teague KA, LaCaze SD, Lawhon D, Varner D. The Effects of antibiotic type and extender storage method on sperm quality and antibacterial effectiveness in fresh and cooled-stored stallion semen. Theriogenology. 2018;122:23-29.

Nattadiputra, S dan S. Munaf. Aminoglikosida dan beberapa antibiotika khusus, kumpulan kuliah farmakologi, 631, Jakarta, EGC; 2009.

Fisher K, Phillips CA. Potential antimicrobial uses of essential oils in food: Is citrus the answer?. Trends in Food Sci and Technology. 2008;19(3):156-164.

Sitepu SA, Marisa J. Percentage value of membrane integrity and acrosome integrity spermatozoa in simmental liquid semen with addition penicillin and sweet orange essential oil. In IOP Conference Series: Earth and Environmental Science. 2019; 327(1):012027. IOP Publishing.

Rodriguezgil JE, Montserrat A, Rigau T. Effects of hypoosmotic incubation on acrosome and tail structure on Canine Spermatozoa. Theriogenology. 1994;42: 815-29.

De Graaf S, Beilby KH, Underwood SL, Evans G, Maxwell WMC. Sperm sexing in sheep and cattle: The exception and the rule. Theriogenology. 2009;71(1):89-9.

El-Sheshtawy RI, Sisy GA, El-Nattat WS. Effects of different concentrations of sucrose or trehalose on the post-thawing quality of cattle bull semen. Asian Pacific Journal of Reproduction. 2015;4(1): 26-31.

Maxwell WMC, Watson PF. Recent progress in the preservation of ram semen. Anim. Reprod. Sci. 1996;42:55-65.

Sinha S, Deka BC, Tamulu MK, Borgohain BN. Effect of equilibration period and glicerol level in tris extender of quality of frozen Goat Semen. Indian Vet. J. 1992; 69:1107-1110.

Hafez ESE, dan B. Hafez. Reproduction in Farm Animal 7th Ed. Lippincott Williams and Walkins, South Carolina; 2000.

Singh VK, Singh AK, Kumar R, Atreja SK. Development of soya milk extender for semen cryopreservation of Karan Fries (crossbreed cattle). CryoLetters. 2013; 34(1):52-61.

Tambing SN, Sutama IK, Sariubang M. Efficacy of concentration of egg yolk in Tris extender with and without seminal plasma on frozen semen quality of Saanen bucks. Jurnal Ilmu Ternak dan Veteriner. 2009; 13(4):315-322.

Xu J, Chaubal SA, Du F. Optimizing IVF with sexed sperm in cattle. Theriogenology. 2009;71(1):39-47.

Bilaspuri GS, Bansal AK. Mn2+: A potent antioxidant and stimulator of sperm capacitation and acrosome reaction in crossbred cattle bulls. Archives Animal Breeding. 2008;51(2):149-158.

Baril G, Leboeuf B, Saumande J. Syncronitation of estrus in goat: The relationship between time of occurrence of estrus and fertility following artificial insemination. Theriogenology. 1993;40:621-628.