In vivo Assessment of Antidiabetic Potential and Mapping of Pharmacological Properties of Ethanolic Extract of Leaves of Coccinia grandis on Alloxan-induced Diabetic Rats

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Zahirul Islam
Md. Rafat Tahsin
Ahmad Ullah Faisal
Tanzia Islam Tithi
Tasnova Tasnim Nova
Tahmina Sharif Nila
Md. Zobayer Hossain Gorapi
Faisal Ahmed Nadvi
Tasneem Nayla Mridula
Abu Asad Choudhury
Jakir Ahmed Chowdhury
Shaila Kabir
Md. Shah Amran


Diabetes is a metabolic disease and plant derived products are used to combat this deadly disease. Plant is a diverse source of numerous therapeutic compounds which can be used to ameliorate diabetes. Leaves of Coccinia grandis (L.) (Family: Cucurbitaceae) is one of the leafy vegetable that is used for this purpose traditionally. It has been used against diabetes for a very long time. Our aims were to identify the hypoglycemic effect of extract of leaves of Coccinia grandis as well as to determine its safety profile so that we could use the plant material to improve the diabetic condition. Diabetes was induced in rats by intraperitoneal injection of alloxan at a dose of 150 mg/Kg bodyweight and ethanolic extract of leaves of Coccinia grandis was fed to the rats at a dose of 750 mg/kg. We measured blood glucose level, and safety profile by measuring SGOT, SGPT and creatinine level on diabetic and non-diabetic rats before and after administration of the extract. After measuring blood glucose level, it was found that the hypoglycemic efficacy was comparable to that of metformin (p> 0.05) which was given at a dose of 500 mg/kg. Safety profile were investigated by checking SGOT, SGPT and creatinine level. It was seen that both metformin and leaf extract of Coccinia grandis improved the pathological condition induced by diabetes. Furthermore, in healthy individual rats both metformin and leaf extract of Coccinia grandis did not significantly alter the normal physiological state. It might, therefore, be inferred that the extract of leaves of Coccinia grandis could be used as a good alternative therapy to treat diabetes.

Coccinia grandis (L.), diabetes, alloxan, rat, safety profile, SGOT, SGPT, creatinine, alternative therapy.

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How to Cite
Islam, Z., Tahsin, M. R., Ullah Faisal, A., Islam Tithi, T., Tasnim Nova, T., Sharif Nila, T., Hossain Gorapi, M. Z., Ahmed Nadvi, F., Nayla Mridula, T., Asad Choudhury, A., Ahmed Chowdhury, J., Kabir, S., & Amran, M. S. (2019). In vivo Assessment of Antidiabetic Potential and Mapping of Pharmacological Properties of Ethanolic Extract of Leaves of Coccinia grandis on Alloxan-induced Diabetic Rats. Asian Journal of Advanced Research and Reports, 7(2), 1-9.
Original Research Article


Wild S, Roglic Green A, Sicree R, King H. Global prevalence of diabetes: Estimates for the year 2000 and projections for 2030. Diabetes Care. 2004;27(5):1047-1053.

WHO - World Health Organization. Global report on diabetes. 1. Diabetes Mellitus - Epidemiology. 2. Diabetes Mellitus - Prevention and control. 3. Diabetes, gestational. 4. Chronic disease. 5. Public health. Geneva: WHO; 2016.

Venkateswaran S, Pari L. Effect of Coccinia indica on blood glucose, insulin and key hepatic enzymes in experimental diabetes. Pharmaceutical Biology. 2002;3: 165-170.

Schaefer H, Heibl C, Renner SS. Gourds afloat: A dated phylogeny reveals an Asian origin of the gourd family (Cucurbitaceae) and numerous oversea dispersal events. Proc Biol Sci. 2009;276(1658):843–851.

Bhattacharya M. A historical exploration of Indian dietsand a possible link to insulin resistance syndrome. Appetite. 2015;95: 421-454.

Ediriweera ERHSS, Ratnasooriya WD. A review of herbs used in diabetes mellitus by Sri Lankan ayurvedic and traditional physicians. Ayurveda. 2009;30:373-391.

Sunilson JAJ, Muthappan M, Das A, Suraj R, Varatharajan R, Promwichit P. Hepatoprotective activity of Coccinia grandis leaves against carbon tetrachloride induced hepatic injury in rats. Int J Pharmacol. 2009;5(3):222-227.

Bole S, Ashwini M, Lather N, Vedamutrhy AB, Balu S. In vitro antioxidant and antiinflammatory activity of Coccinia grandis. Int J Pharm Sci. 2012;4(3):239-242.

Ajay SS. Hypoglycemic activity of Coccinia indica (Cucurbitaceae) leaves. Int J Pharm Tech Res. 2009;1(3):892-893.

Krishnakumari S, Bhuvaneswari P, Rajeswari P. Ameliorative potential of Coccinia grandis extract on serum and liver marker enzymes and lipid profile in streptozotocin induced diabetic rats. Ancient Sci Life. 2011;31(1):26-30.

Bhattacharya Bolay, et al. In vitro evaluation of antifungal and antibacterial activities of the plant Coccinia grandis (L.) Voigt. (Family-Cucurbitaceae). Journal of Phytology. 2010;2(11):52-57.

Bulbul Israt Jahan, Nathan Laizuman, Haque Mahmuda. Antibacterial cytotoxic and antioxidant activity of chloroform, n-hexane and ethyl acetate extract of plant Coccinia cordifolia. Agriculture and Biology Journal of North America. 2011;2(4):713-719.

Sivaraj A, et al. Antibacterial activity of Coccinia grandis leaf extract in selective bacterial strains. Journal of Applied Pharmaceutical Science. 2011;01(07):120-123.

Samanta Amalesh, Bhattacharya Bolay, Ghosh Soma, Gouranga Das. In vivo antimalarial activity of the plant Coccinia grandis. International Journal of Pharma-ceutical Research and Development. 2011;3(4):73-79.

Rahumann AA. Larvicidal efficacy of five plant leaf extract against mosquito specious. Journal of Paracitol Research. 2008;103:133-139.

Tamilselvan N, et al. Pharmacognosy of Coccinia grandis: A review. Asian Pacific Journal of Tropical Biomedicine. 2011;1(1): S299-S302.

Behera SK, Dash V. Some Indian vegetable used as an anticancer agent. International Journal of Advanced Pharmaceutical and Biological Sciences. 2012;2:250-264.

Nanasombat S, Teckchuen N. Anti-microbial, antioxidant and anticancer activities of Thai local vegetables. Journal of Medicinal Plants Research. 2009;3950: 443-449.

Aggarwal Ashish S, et al. Analgesic and antipyretic activity of methanolic extract of Coccinia grandis L. leaves in experimental animals. Research Journal of Pharmaceutical, Biological and Chemical Sciences. 2011;2:175-182.

Manoharan Preeth, John Shobana, Golla Upendarrao, Thangathirupathi A. Antiulcer effect of Coccinia grandis on pylorus grandis on pylorus ligated albino rats. International Journal of Pharma Research and Development. 2010;2:1-9.

Girish C, et al. Evaluation of antiulcer activity of Coccinia grandis leaves. Research Journal of Pharmacology and Pharmacodynamics. 2011;3:2011.

Pattanayak Shakti Prasad, Priyashree Sunita. In vivo antitussive activity of Coccinia grandis against irritant aerosol and sulfur dioxide-induced cough model in rodents. Bangladesh Journal of Pharmacology. 2009;4:84-87.

Singha Geetu, et al. Antidyslipidemic activity of polyprenol from Coccinia grandis in high-fat diet-fed hamster model. Phytomedicine. 2007;14:792-798.

Bhuiyan MN, et al. Mutagenic effect of Coccinia cordifolia leaf extract on N. crassa fungus. Bangladesh Journal of Science and Research. 2009;44:215-220.

Jaiboon Vareerat, Boonyanuphap Jaruntorn, Sajee Suwansri, Puntarika Ratanatraiwong, Chanida Hansawasdi. Alpha amylase inhibition and roasting time of local vegetables and herbs prepared for diabetes risk reducing chili paste. Asian Journal of Food and Agro-Industry. 2011;14(02):03-113.

Al-Amin Md, Uddin Mir Muhammad Nasir, Rizwan Ashfique, Islam Md. Effect of ethanol extract of Coccinia grandis Lin leaf on glucose and cholesterol lowering activity. British Journal of Pharmaceutical Research. 2013;3:1070-1078. ISSN: 2231-2919.

Hassan Zurina, Yam Mun, Ahmad Mariam, Yusof Ahmad. Antidiabetic properties and mechanism of action of Gynura procumbens water extract in streptozotocin-induced diabetic rats. Molecules (Basel, Switzerland). 2010;15: 9008-23.
DOI: 10.3390/molecules15129008

Attanayake Anoja, Jayatilaka KAPW, Pathirana Chitra, Mudduwa Lakmini. Antihyperglycemic activity of Coccinia grandis (L.) Voigt in streptozotocin induced diabetic rats. Indian Journal of Traditional Knowledge. 2015;14:376-381.

Yin Peipei, Wang Yu, Yang Lingguang, Sui Jinling, Liu Yujun. Hypoglycemic effects in alloxan-induced diabetic rats of the phenolic extract from Mongolian Oak Cups enriched in ellagic acid, kaempferol and their derivatives. Molecules. 2018;23: 1046.
DOI: 10.3390/molecules23051046

Sharma SB, Nasir A, Prabhu KM, Murthy PS, Dev G. Hypoglycaemic and hypolipidemic effect of ethanolic extract of seeds Eugenia jambolana in alloxan-induced diabetic rats. J. Ethnopharmacol. 2003;85:201-206.

Rösen P, Wiernsperger NF Metformin delays the manifestation of diabetes and vascular dysfunction in Goto-Kakizaki rats by reduction of mitochondrial oxidative stress. Diabetes Metab Res Rev. 2006;22(4):323-30.